Sample Requirements for Biopharma / Research Services

Biopharma / Research Resources

Sample Requirements for Biopharma / Research Services

Sample and Shipping requirements are determined by the sample and service type. If your samples don’t meet the listed criteria, reach out to us. We’ll do our best to create a tailored solution that suits your needs.

DNA Extraction

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

Whole blood

Recommended: 3ml
Minimum: 1ml

No clotting

EDTA

Dry ice

Saliva

2ml

N/A

Oragene OG500/OG600 Tube Additive

Ambient temperature

PBMCs

1ml, ≥10 million cells

N/A

Cryopreservative

Dry ice

Buffy Coat

300µl - 1ml

No clotting

EDTA

Dry ice

FFPE Tissue

1 H&E slide and 10 unstained sections (5-10 microns) or 2mm³

Ensure sections are uniform in thickness and do not contain excessive folds or artifacts

N/A

Ambient temperature

Snap Frozen Tissue

20-50mg

N/A

Dry ice

Bone Marrow Aspirate

1-2ml

N/A

EDTA

Dry ice

RNA Extraction

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

Whole blood
(PAXgene)

2.5ml whole blood in 6.9ml additive
(PAXgene)

No clotting

6.9ml additive

Fridge (2-8°C) /
Ambient temperature

PBMCs

≥1ml , ≥10 million cells

N/A

Cryopreservative

Dry ice

FFPE Tissue

1 H&E slide and 10 unstained sections (5-10 microns) or 2mm³

Ensure sections are uniform in thickness and do not contain excessive folds or artifacts

N/A

Ambient temperature

Snap Frozen Tissue

20-50mg

N/A

Dry ice

Bone Marrow Aspirate

1-2ml

N/A

EDTA

Dry ice

Genotyping

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

DNA

Concentration: ≥20ng/µl Volume: ≥60ul

Purity (OD 260/280 nm): 1.7 - 2.0 Integrity (DIN): ≥7

Fridge (2-8°C) /
Ambient temperature

Whole blood

Recommended: 3ml
Minimum: 1ml

No clotting

EDTA

Dry ice

Saliva

2ml

N/A

Oragene OG500/OG600 Tube Additive

Ambient temperature

Buffy Coat

300µl - 1ml

No clotting

EDTA

Dry ice

PBMCs

≥1ml, ≥10 million cells

N/A

Cryopreservative

Dry ice

Methylation Profiling

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

DNA

Concentration: ≥25ng/µl Volume: ≥110ul

Purity (OD 260/280 nm): 1.7 - 2.0 Integrity (DIN): ≥7

Fridge (2-8°C) /
Ambient temperature

FFPE DNA

Concentration: ≥25ng/µl Volume: ≥110µl

Purity (OD 260/280 nm): 1.7 - 2.0
Recommended Integrity (DIN): ≥5

Fridge (2-8°C) /
Ambient temperature

Whole blood

Recommended: 3ml
Minimum: 1ml

No clotting

EDTA

Dry ice

Saliva

2ml

N/A

Oragene OG500/OG600 Tube Additive

Ambient temperature

PBMCs

≥1ml, ≥10 million cells

N/A

Cryopreservative

Dry ice

Snap Frozen Tissue

20-50mg

N/A

Dry ice

FFPE Tissue

1 H&E slide and 10 unstained sections (5-10 microns) or 2mm³

Ensure sections are uniform in thickness and do not contain excessive folds or artifacts

N/A

Ambient temperature

Whole Genome Sequencing

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

DNA

Concentration: ≥20ng/µl Recommended Volume: ≥130µl
Minimum Volume: ≥70µl

Purity (OD 260/280 nm): 1.7 - 2.0 Integrity (DIN): ≥7

Fridge (2-8°C) /
Ambient temperature

Whole blood

Recommended: 3ml
Minimum: 1ml

No clotting

EDTA

Dry ice

Saliva

2ml

N/A

Oragene OG500/OG600 Tube Additive

Ambient temperature

Buffy Coat

300µl - 1ml

No clotting

EDTA

Dry ice

PBMCs

≥1ml, ≥10 million cells

N/A

Cryopreservative

Dry ice

Whole Exome Sequencing

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

DNA

Concentration: 10-1000ng/µl Volume: ≥50µl

Purity (OD 260/280 nm): 1.7 - 2.0 Integrity (DIN): ≥7

Fridge (2-8°C) /
Ambient temperature

Whole blood

Recommended: 3ml
Minimum: 1ml

No clotting

EDTA

Dry ice

Snap Frozen Tissue

20-50mg

N/A

Dry ice

Sanger Sequencing

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

DNA

Concentration: 10-1000ng/µl Volume: ≥50µl

Purity (OD 260/280 nm): 1.7 - 2.0 Integrity (DIN): ≥7

Fridge (2-8°C) /
Ambient temperature

Whole blood

Recommended: 3ml
Minimum: 1ml

No clotting

EDTA

Dry ice

Total RNA Sequencing

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

RNA

Concentration: ≥10ng/µl Volume: ≥30ul

Integrity (RIN): ≥7

Dry ice

Whole blood
(PAXgene)

Recommended: 2.5ml whole blood in 6.9ml additive (PAXgene)

N/A

6.9ml additive

Fridge (2-8°C) /
Ambient temperature

PBMCs

≥1ml, ≥10 million cells

N/A

Cryopreservative

Dry ice

Snap Frozen Tissue

20-50mg

N/A

Dry ice

Single Cell Immune Profiling

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

PBMCs

≥1ml, ≥10 million cells

≥80% viability

Cryopreservative

Recommended: Shipping vapour phase.
Minimum: Dry ice

Single Cell Gene Expression

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

PBMCs

≥1ml, ≥10 million cells

≥80% viability

Cryopreservative

Recommended: Shipping vapour phase.
Minimum: Dry ice

Single Nuclei Gene Expression

Sample Type

Quantity

Quality

Collection Buffer

Shipping Conditions

Snap Frozen Liver Tissue

Minimum: 50mg
Recommended: 125mg

N/A

Dry ice